Open AccessInvestigation into the prevalence of a novel dendritic‐like cell subset in vivo
Author(s) -
Griffiths Kristin Lisa,
Tan Jonathan Kah Huat,
O'Neill Helen Christine
Publication year - 2013
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12174
Subject(s) - in vivo , biology , cell , dendritic cell , microbiology and biotechnology , computational biology , immunology , genetics , immune system
A novel dendritic‐like cell subset termed L‐ DC was recently identified in murine spleen based on marker expression of a homogeneous cell population derived from long‐term culture of neonatal spleen. The function of L‐ DC is distinct from other splenic dendritic and myeloid cell subsets because of their high endocytic capacity and their ability to cross‐present antigen to CD 8 + T cells. This paper shows the subset to be unique to spleen and blood, with a similar, but possibly functionally distinct subset also present in bone marrow. The prevalence of the subset is low; ~6% of all dendritic and myeloid cells in the spleen and ~5% in blood. However, they are a distinct cell type on the basis of marker expression, and endocytic and T‐cell stimulatory capacity. Attempts to identify an enriched population of these cells in mutant mouse strains with reported increases in myelopoiesis showed either a lack of L‐ DC or an altered phenotype reflective of the phenotype of the mouse strain.