Open AccessAn intensified systemic trafficking of bone marrow‐derived stem/progenitor cells in patients with pancreatic cancer
Author(s) -
Starzyńska Teresa,
Dąbkowski Krzysztof,
Błogowski Wojciech,
ZubaSurma Ewa,
Budkowska Marta,
Sałata Daria,
Dołęgowska Barbara,
Marlicz Wojciech,
Lubikowski Jerzy,
Ratajczak Mariusz Z.
Publication year - 2013
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12065
Subject(s) - progenitor cell , bone marrow , pancreatic cancer , cancer research , cancer , stem cell , medicine , progenitor , biology , pathology , microbiology and biotechnology
Various experimental studies indicate potential involvement of bone marrow ( BM )‐derived stem cells ( SC s) in malignancy development and progression. In this study, we comprehensively analysed systemic trafficking of various populations of BM‐derived SCs ( BMSC s), i.e ., mesenchymal, haematopoietic, endothelial stem/progenitor cells ( MSC s, HSC s, EPC s respectively), and of recently discovered population of very small embryonic/epiblast‐like SC s ( VSEL s) in pancreatic cancer patients. Circulating CD 133 + /Lin − / CD 45 − / CD 34 + cells enriched for HSC s, CD 105 + / STRO ‐1 + / CD 45 − cells enriched for MSC s, CD 34 + / KDR + / CD 31 + / CD 45 − cells enriched for EPC s and small CXCR 4 + CD 34 + CD 133 + subsets of Lin − CD 45 − cells that correspond to VSEL s were enumerated and sorted from blood samples derived from 29 patients with pancreatic cancer, and 19 healthy controls. In addition, plasma levels of stromal‐derived factor‐1 ( SDF ‐1), growth/inhibitory factors and sphingosine‐1‐phosphate (S1P; chemoattractants for SC s), as well as, of complement cascade ( CC ) molecules (C3a, C5a and C5b‐9/membrane attack complex – MAC ) were measured. Higher numbers of circulating VSEL s and MSC s were detected in pancreatic cancer patients ( P < 0.05 and 0.01 respectively). This trafficking of BMSC s was associated with significantly elevated C5a ( P < 0.05) and C5b‐9/ MAC ( P < 0.005) levels together with S1P concentrations detected in plasma of cancer patients, and seemed to be executed in a SDF ‐1 independent manner. In conclusion, we demonstrated that in patients with pancreatic cancer, intensified peripheral trafficking of selected populations of BMSC s occurs. This phenomenon seems to correlate with systemic activation of the CC , hepatocyte growth factor and S1P levels. In contrast to previous studies, we demonstrate herein that systemic SDF ‐1 levels do not seem to be linked with increased mobilization of stem cells in patients with pancreatic cancer.