Open AccessChanges of lysosomes in the earliest stages of the development of atherosclerosis
Author(s) -
Bobryshev Yuri V.,
Shchelkunova Tatyana A.,
Morozov Ivan A.,
Rubtsov Petr M.,
Sobenin Igor A.,
Orekhov Alexander N.,
Smirnov Alexander N.
Publication year - 2013
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12042
Subject(s) - fatty streak , lysosome , immunocytochemistry , biology , arteriosclerosis , oil red o , endosome , phagocytosis , acid phosphatase , pathology , microbiology and biotechnology , endocrinology , cholesterol , enzyme , biochemistry , gene , medicine , intracellular , adipogenesis
One of hypotheses of atherosclerosis is based on a presumption that the zones prone to the development of atherosclerosis contain lysosomes which are characterized by enzyme deficiency and thus, are unable to dispose of lipoproteins. The present study was undertaken to investigate the characteristics and changes of lysosomes in the earliest stages of the development of atherosclerosis. Electron microscopic immunocytochemistry revealed that there were certain changes in the distribution of CD68 antigen in lysosomes along the ‘normal intima‐initial lesion‐fatty streak’ sequence. There were no significant changes found in the key mRNA s encoding for the components of endosome/lysosome compartment in initial atherosclerotic lesions, but in fatty streaks, the contents of EEA 1 and Rab5a mRNA s were found to be diminished while the contents of CD 68 and p62 mRNA s were increased, compared with the intact tissue. The study reinforces a view that changes occurring in lysosomes play a role in atherogenesis from the very earlier stages of the disease.