Open AccessFrequency of angiotensin II type 1 receptor gene polymorphism in Turkish acute stroke patients
Author(s) -
Hulyam Kurt,
Aysegul Bayramoglu,
Veysi Gunes Hasan,
Demet Ozbabalik,
Irfan Degirmenci,
Ertugrul Colak,
Didem Cosan Turgut,
Banu Bayram,
Miris Dikmen
Publication year - 2013
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12017
Subject(s) - turkish population , genotype , allele frequency , restriction fragment length polymorphism , allele , gene polymorphism , agarose gel electrophoresis , polymorphism (computer science) , polymerase chain reaction , gastroenterology , medicine , genomic dna , angiotensin ii , biology , microbiology and biotechnology , gene , genetics , receptor
This study was performed in acute stroke patients in the Turkish population to determine the frequency of the A1166C polymorphism in the AT 1 gene and to examine the role of this polymorphism in acute stroke development. In this study, 257 genomic DNA samples were analysed (from 206 acute stroke patients and 51 healthy individuals). Genomic DNA was prepared from peripheral blood using the salt‐extraction method. The presence of the A1166C polymorphism in the AT 1 gene was determined using the polymerase chain reaction ( PCR )‐restriction fragment length polymorphism ( RFLP ) method. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge‐coupled device ( CCD ) camera. In this study, the allele frequency at the A1166C position was 92% A and 8% C for control and 97% A and 3% C for patients. This difference in allele frequency between the control group and the patient group was not statistically significant. However, genotype and allele frequencies showed a significant difference ( P < 0.001) in the control and the patient groups. The results of this study show no relationship between the A1166C polymorphism in the AT 1 gene and acute stroke in the Turkish population.