Open AccessInfiltration of myeloid cells into decidua is a critical early event in the labour cascade and post‐partum uterine remodelling
Author(s) -
Shynlova Oksana,
NeddRoderique Tamara,
Li Yunqing,
Dorogin Anna,
Nguyen Tina,
Lye Stephen J.
Publication year - 2013
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/jcmm.12012
Subject(s) - decidua , chemokine , infiltration (hvac) , biology , andrology , flow cytometry , myeloid , placenta , medicine , endocrinology , immunology , inflammation , pregnancy , fetus , genetics , physics , thermodynamics
Leucocyte infiltration in the decidua (maternal–foetal interface) before, during and after term ( TL ) and preterm labour ( PTL ) was studied in mouse. We also investigated the mechanism of peripheral leucocyte recruitment into decidua by analysing the tissue cytokine profiles. Decidual tissues were collected during late gestation, TL and post‐partum ( PP ). PTL was initiated on gestational day 15 by intrauterine injection of Lipopolysaccharide ( LPS , 125 μg) or progesterone signalling antagonism by RU 486. Animals were killed during PTL or PP . Decidua basalis was analysed using FACS and immunohistochemistry. Markers of myeloid cell differentiation (Gr1, Ly6G, Neu7/4, F4/80) were assessed to define tissue monocytes ( M ), neutrophils ( N ) and macrophages (Macs). Flow cytometry revealed a significant ( P < 0.05) increase in decidual Macs prior to TL ; M and N numbers increased during TL and further increased during PP , which correlated with immunohistochemistry data. Massive influx of N , but not Macs and M , was detected by FACS during LPS ‐ PTL ( P < 0.05) but not RU 486‐ PTL . Highest levels of N infiltration into the decidua occurred PP in both LPS and RU 486 groups. Decidual infiltration during TL and RU486‐PTL was accompanied by an increase in pro‐inflammatory cytokines ( IL 1b and IL 6) and CCL 2 chemokine; LPS ‐ PTL showed increases in multiple cytokines. PP period following TL and PTL was associated with further up‐regulation of multiple cytokines/chemokines ( P < 0.05). Our data suggest a programme of myeloid cells involvement in parturition with the pre‐partum influx of Macs into the decidua contributing to the progression of labour, whereas the later influx of M and N contribute to PP decidual involution.