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Across‐breed validation study confirms and identifies new loci associated with sexual precocity in Brahman and Nellore cattle
Author(s) -
Melo Thaise Pinto,
Salinas Fortes Marina Rufino,
Hayes Ben,
Albuquerque Lucia Galvão,
Carvalheiro Roberto
Publication year - 2020
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/jbg.12429
Subject(s) - brahman , snp , biology , candidate gene , single nucleotide polymorphism , genetics , breed , population , snp array , gene , genotype , medicine , environmental health
The aim of this study was to identify candidate regions associated with sexual precocity in Bos indicus . Nellore and Brahman were set as validation and discovery populations, respectively. SNP selected in Brahman to validate in Nellore were from gene regions affecting reproductive traits (G1) and significant SNP ( p ≤ 10 –3 ) from a meta‐analysis (G2). In the validation population, early pregnancy (EP) and scrotal circumference (SC) were evaluated. To perform GWAS in validation population, we used regression and Bayes C. SNP with p ≤ 10 –3 in regression and Bayes factor ≥3 in Bayes C were deemed significant. Significant SNP (for EP or SC) or SNP in their ±250 Kb vicinity region, which were in at least one discovery set (G1 or G2), were considered validated. SNP identified in both G1 and G2 were considered candidate. For EP, 145 SNP were validated in G1 and 41 in G2, and for SC, these numbers were 14 and 2. For EP, 21 candidate SNP were detected (G1 and G2). For SC, no candidate SNP were identified. Validated SNP and their vicinity region were located close to quantitative trait loci or genes related to reproductive traits and were enriched in gene ontology terms related to reproductive success. These are therefore strong candidate regions for sexual precocity in Nellore and Brahman.