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Mitochondrial DNA variation of indigenous goats in N arok and I siolo counties of K enya
Author(s) -
Kibegwa F.M.,
Githui K.E.,
Jung'a J.O.,
Badamana M.S.,
Nyamu M.N.
Publication year - 2016
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/jbg.12182
Subject(s) - mitochondrial dna , indigenous , biology , zoology , veterinary medicine , genetics , gene , medicine , ecology
Summary Phylogenetic relationships among and genetic variability within 60 goats from two different indigenous breeds in N arok and I siolo counties in K enya and 22 published goat samples were analysed using mitochondrial control region sequences. The results showed that there were 54 polymorphic sites in a 481‐bp sequence and 29 haplotypes were determined. The mean haplotype diversity and nucleotide diversity were 0.981 ± 0.006 and 0.019 ± 0.001, respectively. The phylogenetic analysis in combination with goat haplogroup reference sequences from G en B ank showed that all goat sequences were clustered into two haplogroups ( A and G ), of which haplogroup A was the commonest in the two populations. A very high percentage (99.90%) of the genetic variation was distributed within the regions, and a smaller percentage (0.10%) distributed among regions as revealed by the analysis of molecular variance ( amova ). This amova results showed that the divergence between regions was not statistically significant. We concluded that the high levels of intrapopulation diversity in I siolo and N arok goats and the weak phylogeographic structuring suggested that there existed strong gene flow among goat populations probably caused by extensive transportation of goats in history.