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Characterization of micro RNA profile in mammary tissue of dairy and beef breed heifers
Author(s) -
Wicik Z.,
Gajewska M.,
Majewska A.,
Walkiewicz D.,
Osińska E.,
Motyl T.
Publication year - 2016
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/jbg.12172
Subject(s) - microrna , biology , mammary gland , wnt signaling pathway , gene , gene expression , microarray analysis techniques , microarray , real time polymerase chain reaction , andrology , microbiology and biotechnology , endocrinology , medicine , genetics , cancer , breast cancer
Summary Micro RNA s (mi RNA s) are small non‐coding RNA s that participate in the regulation of gene expression. Their role during mammary gland development is still largely unknown. In this study, we performed a microarray analysis to identify mi RNA s associated with high mammogenic potential of the bovine mammary gland. We identified 54 significantly differentially expressed mi RNA s between the mammary tissue of dairy (Holstein‐Friesian, HF ) and beef (Limousin, LM ) postpubertal heifers. Fifty‐two mi RNA s had higher expression in the mammary tissue of LM heifers. The expression of the top candidate mi RNA s (bta‐miR‐10b, bta‐miR‐29b, bta‐miR‐101, bta‐miR‐375, bta‐miR‐2285t, bta‐miR‐146b, bta‐let7b, bta‐miR‐107, bta‐miR‐1434‐3p) identified in the microarray experiment was additionally evaluated by qPCR . Enrichment analyses for targeted genes revealed that the major differences between mi RNA expression in the mammary gland of HF versus LM were associated with the regulation of signalling pathways that are crucial for mammary gland development, such as TGF ‐beta, insulin, WNT and inflammatory pathways. Moreover, a number of genes potentially targeted by significantly differentially expressed mi RNA s were associated with the activity of mammary stem cells. These data indicate that the high developmental potential of the mammary gland in dairy cattle, leading to high milk productivity, depends also on a specific mi RNA expression pattern.