Premium
A genome‐wide association study for canine cryptorchidism in Siberian Huskies
Author(s) -
Zhao X.,
Onteru S.,
Saatchi M.,
Garrick D.,
Rothschild M.
Publication year - 2014
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/jbg.12064
Subject(s) - biology , haplotype , gubernaculum , candidate gene , genome wide association study , genetics , population , snp , genetic association , single nucleotide polymorphism , gene , allele , anatomy , genotype , medicine , environmental health
Summary Cryptorchidism is a condition whereby one or both testes fail to descend into the scrotal sac. Here, we performed a genome‐wide association study (GWAS) with both a case–control analysis using the GEMMA software accounting for population structure and a BayesB approach in the GenSel software applied to every 1 Mb window of SNP s or haplotypes. The haplotypes were constructed from a genealogical tree using the population of 204 Siberian Huskies. The BayesB analyses identified six putative genomic candidate regions on CFA 6, 9, 24, 27 and X. These regions explained a high percentage of genetic variance when compared with other genomic regions. The positional candidate genes Q9 TSI 5_ CANFA (matrix metalloproteinase 9 precursor) on CFA 24, ADAMTS 20 ( ADAM metallopeptidase with thrombospondin type 1 motif, 20) on CFA 27 and MID 1 IP 1 ( MID 1 interacting protein 1) on CFAX are known to be functionally related to extracellular matrix remodelling, which might be important for gubernaculum elongation and thus interrupting normal testicular descent. Further mutation screening in these candidate regions on CFA 6, 9, 24, 27 and X is needed. Next generation sequencing will help to uncover rare variants associated with cryptorchidism in this dog population.