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Polymorphisms in the promoter region of myostatin gene are associated with carcass traits in pigs
Author(s) -
Tu P.A.,
Lo L.L.,
Chen Y.C.,
Hsu C.C.,
Shiau J.W.,
Lin E.C.,
Lin R.S.,
Wang P.H.
Publication year - 2014
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/jbg.12053
Subject(s) - myostatin , biology , carcass weight , zoology , body weight , medicine , skeletal muscle , endocrinology
Summary Higher average daily gain, more lean meat yield and less fat yield of porcine carcass increase selling profits for animal producers. Myostatin ( MSTN ), previously called GDF 8, is a member of transforming growth factor‐β ( TGF ‐β) superfamily. It is a negative regulator for both embryonic development and adult homeostasis of skeletal muscle. In this study, the genotypes of the previously described SNP s MSTN g.435 G > A and g.447 A > G SNP s in 66 D uroc pigs, 33 L andrace pigs, 180 D uroc ×  L andrace ( DL ) pigs and 155 D uroc ×  Y orkshire ×  L andrace ( DYL ) pigs were determined by T aqman SNP G enotyping A ssays. For D uroc and L andrace pigs, MSTN g.435 GG /g.447 AA individual had greater backfat thickness (p < 0.05) than g.435 AA /g.447 GG individual, whereas MSTN g.435 AA /g.447 GG had greater meat (p < 0.05) and meat percentage (p < 0.05) than g.435 GA /g.447 AG individual. For DL and DYL pigs, the MSTN g.435 GG /g.447 AA animals were greater in backfat at ultrasound 10th rib (p < 0.05) and carcass 10th rib (p < 0.01) than g.435 AA /g.447 GG individual. The MSTN g.435 AA /g.447 GG individual also had higher values than g.435 GG /g.447 AA for anterior‐end meat (p < 0.05), posterior‐end meat (p < 0.01), total meat weight (p < 0.01) and meat percentage (p < 0.01). This study confirmed evidence that MSTN g.435 G > A and g.447 A > G affected carcass traits in pigs. The effects of the mutated alleles were additive with the maximal effects resulting from two copies of the mutated allele. Selection for MSTN g.435 A /g.447 G allele is expected to increase muscle of limb and total meat production and decrease backfat thickness.

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