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Efficacy of EPA ‐registered disinfectants against two human norovirus surrogates and Clostridioides difficile endospores
Author(s) -
Huang Jinge,
Park Geun Woo,
Jones Rachael M.,
Fraser Angela M.,
Vinjé Jan,
Jiang Xiuping
Publication year - 2022
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.15524
Subject(s) - endospore , microbiology and biotechnology , norovirus , feline calicivirus , murine norovirus , clostridioides , log reduction , disinfectant , chemistry , biology , virology , spore , virus , organic chemistry
Aims To determine the efficacy of a panel of nine EPA‐registered disinfectants against two human norovirus (HuNoV) surrogates (feline calicivirus [FCV] and Tulane virus [TuV]) and Clostridioides difficile endospores. Methods and Results Nine EPA‐registered products, five of which contained H 2 O 2 as active ingredient, were tested against infectious FCV, TuV and C. difficile endospores using two ASTM methods, a suspension and carrier test. Efficacy claims against FCV were confirmed for 8 of 9 products. The most efficacious product containing H 2 O 2 as ingredient achieved a >5.1 log reduction of FCV and >3.1 log reduction of TuV after 5 min, and >6.0 log reduction of C. difficile endospores after 10 min. Of the five products containing H 2 O 2 , no strong correlation ( R 2 = 0.25, p = 0.03) was observed between disinfection efficacy and H 2 O 2 concentration. Addition of 0.025% ferrous sulphate to 1% H 2 O 2 solution improved efficacy against FCV, TuV and C. difficile . Conclusion Disinfectants containing H 2 O 2 are the most efficacious disinfection products against FCV, TuV and C. difficile endospores. Product formulation, rather than the concentration of H 2 O 2 in a product, impacts the efficacy of a disinfection product. Significance and Impact of Study H 2 O 2 ‐based disinfectants are efficacious against surrogate viruses for HuNoV and C. difficile endospores.