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Plant endophytic bacteria: A potential resource pool of electroactive micro‐organisms
Author(s) -
Ling Lijun,
Yang Caiyun,
Li Zibin,
Luo Hong,
Feng Shenglai,
Zhao Yunhua,
Lu Lu
Publication year - 2022
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.15368
Subject(s) - bacteria , biology , microbial fuel cell , botany , inoculation , microbiology and biotechnology , paenibacillus , bacillus (shape) , chemistry , anode , horticulture , genetics , 16s ribosomal rna , electrode
Aims Electroactive micro‐organisms play a significant role in microbial fuel cells. It is necessary to discover potential resources in plant endophytes. In this study, plant tissues were selected to isolate endophytic bacteria, and the electrochemical activity potential was evaluated. Methods and results The microbial fuel cell (MFC) is used to evaluate the electricity‐producing activity of endophytic bacteria in plant tissues, and the species distribution of micro‐organisms in the anode of the MFC after inoculation of plant tissues is determined by high‐throughput sequencing. Twenty‐six strains of bacteria were isolated from plant tissues belonging to Angelica and Sweet Potato, of which 17 strains from six genera had electrochemical activity, including Bacillus sp., Pleomorphomonas sp., Rahnella sp., Shinella sp., Paenibacillus sp. and Staphylococcus sp. Moreover, the electricity‐producing micro‐organisms in the plant tissue are enriched. Pseudomonas and Clostridioides are the dominant genera of MFC anode inoculated with angelica tissue. Staphylococcus and Lachnoclostridium are the dominant genera in MFC anode inoculated with sweet potato tissue. And the most representative Gram‐positive strain Staphylococcus succinus subsp. succinus H6 and plant tissue were further analysed for electrochemical activity. And a strain numbered H6 and plant tissue had a good electrogenerating activity. Conclusion This study is of great significance for expanding the resource pool of electricity‐producing micro‐organisms and tapping the potential of plant endophytes for electricity‐producing. Significance and Impact of Study This is the first study to apply plant endophytes to MFC to explore the characteristics of electricity production. It is of great significance for exploring the diversity of plant endophytes and the relationship between electricity producing bacteria and plants.