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In vitro evaluation of anticancer effects of different probiotic strains on HCT‐116 cell line
Author(s) -
Yenuganti V.R.,
Yadala R.,
Azad R.,
Singh S.,
Chiluka V.,
Ahire J.,
Reddanna P.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.15060
Subject(s) - probiotic , microbiology and biotechnology , lactobacillus salivarius , biology , lactobacillus , bifidobacterium bifidum , lactobacillus plantarum , saccharomyces boulardii , lactobacillus reuteri , bifidobacterium , mtt assay , apoptosis , food science , bacteria , biochemistry , fermentation , lactic acid , genetics
Aim Since the evolution of man, microbes are associated with humans, playing a vital role in the maintenance of good health. However, an imbalance in the gut microbial ecosystem is associated with several diseases including colorectal cancer (CRC). The supplementation with probiotics has been proven to be beneficial in improving CRC. In this study, we have evaluated the anticancer effects of 11 probiotic strains on human colorectal carcinoma cell line (HCT‐116). Methods and Results In this study, HCT‐116 cells were treated with various concentrations (0·5, 5, 10, 20 and 200 million CFU per ml) of probiotic strains. The viability was analysed using a MTT assay and IC 50 values were determined. Besides this, we evaluated the expression of multiple genes involved in the apoptosis and stress tolerance by real‐time PCR. Lactobacillus reuteri (UBLRu‐87), Saccharomyces boulardii (Unique‐28), Bacillus clausii (UBBC‐07), Bacillus coagulans (Unique‐IS2), Streptococcus salivarius (UBSS‐01), Lactobacillus fermentum (UBLF‐31), Lactobacillus salivarius (UBLS‐22), Bifidobacterium bifidum (UBBB‐55) and Lactobacillus plantarum (UBLP‐40) exhibited potent cytotoxicity on HCT 116 cells. Furthermore, UBLF‐31 and Unique‐28 induced the expression of CJUN , CFOS and CASP‐9 , and downregulated the expression of BCL6 . UBLRu‐87 and UBBB‐55 induced the expression of CJUN , CFOS and CASP‐9 but not BCL‐6 . UBLP‐40, UBBC‐07, UBLS‐22, and Unique‐IS2 induced the expression of CJUN and CASP‐9 and downregulated the expression of BCL‐6 . Conclusion These studies indicate the anticancer effects of selected probiotic strains by inducing apoptosis. Significance and Impact of the Study The probiotic strains with the anticancer effects identified in this study can be proposed as potential candidates in the treatment of CRCs.