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Antimicrobial activity of the volatile compound 3,5‐dichloro‐4‐methoxybenzaldehyde, produced by the mushroom Porostereum spadiceum , against plant‐pathogenic bacteria and fungi
Author(s) -
Hamamoto E.,
Kimura N.,
Nishino S.,
Ishihara A.,
Otani H.,
OsakiOka K.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.15020
Subject(s) - clavibacter michiganensis , ralstonia solanacearum , antimicrobial , pathogenic bacteria , mycelium , mushroom , biology , bacteria , microbiology and biotechnology , colletotrichum , pathogenic fungus , germination , food science , botany , fungus , pathogen , genetics
Aims In this study, volatile compounds released from mycelia of some aromatic mushrooms were investigated for their inhibitory activity against plant‐pathogenic bacteria and fungi. Methods and Results A screening revealed that volatile compounds from mycelia of Porostereum spadiceum remarkably inhibited the colony formation of plant‐pathogenic bacteria, including Clavibacter michiganensis subsp. michiganensis and Ralstonia solanacearum while also inhibiting the conidial germination of plant‐pathogenic fungi including Alternaria brassicicola and Colletotrichum orbiculare . The volatile compounds were isolated from the culture filtrate of P. spadiceum , and 3,4‐dichloro‐4‐methoxybenzaldehyde (DCMB) was identified as a major compound. DCMB significantly inhibited bacterial colonization at 10 μ g ml −1 and fungal conidial germination at 0·1–1 μ g ml −1 as a vapour. Conclusions This is the first report on the production of the volatile compound DCMB by P. spadiceum and on the antimicrobial activity of DCMB against plant‐pathogenic bacteria and fungi at low concentrations. It may be possible to use the compound as an agent for protecting crops from bacterial and fungal diseases during cultivation and storage. Significance and Impact of the Study This study provides an understanding of antimicrobial activity of the mushroom volatile compound that may be useful as a novel biological control agent for protecting various plant diseases.