Premium
Induction of pre‐chorismate, jasmonate and salicylate pathways by Burkholderia sp. RR18 in peanut seedlings
Author(s) -
Patel Ravikumar R.,
Patel Disha D.,
Bhatt Jaimika,
Thakor Parth,
Triplett Lindsay R.,
Thakkar Vasudev R.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.15019
Subject(s) - jasmonic acid , burkholderia , biology , salicylic acid , rhizosphere , microbiology and biotechnology , bacteria , biochemistry , genetics
Aims To characterize the mechanisms by which bacteria in the peanut rhizosphere promote plant growth and suppress Aspergillus niger , the fungus that causes collar rot of peanut. Methods and Results In all, 131 isolates cultured from the peanut rhizosphere were assayed for growth promotion in a seedling germination assay. The most effective isolate, RR18, was identified as Burkholderia sp. by 16S sequencing analysis. RR18 reduced collar rot disease incidence and increased the germination rate and biomass of peanut seeds, and had broad‐spectrum antifungal activity. Quantitative analyses showed that RR18 induced long‐lasting accumulation of jasmonic acid, salicylic acid and phenols, and triggered the activity of six defence enzymes related to these changes. Comparative proteomic analysis of treated and untreated seedlings revealed a clear induction of four abundant proteins, including a member of the pre‐chorismate pathway, a regulator of clathrin‐coated vesicles, a transcription factor and a hypothetical protein. Conclusion Burkholderia sp. RR18 promotes peanut growth and disease resistance, and stably induces two distinct defence pathways associated with systemic resistance. Significance and Impact of the Study This study demonstrates that a strain of the Burkholderia cepacia complex can elicit both salicylic‐ and jasmonic‐acid‐mediated defences, in addition to having numerous other beneficial properties.