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Investigating the anti‐inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 on LPS‐stimulated PMA‐differentiated THP‐1 cells
Author(s) -
Johari B.,
Maghsood F.,
Madanchi H.,
Moradi M.,
Kadivar M.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14984
Subject(s) - lipopolysaccharide , microbiology and biotechnology , inflammation , biology , interleukin , chemistry , tumor necrosis factor alpha , thp1 cell line , bacteria , lactobacillus reuteri , cytokine , immunology , cell culture , probiotic , genetics
Aims This study was done to investigate the anti‐inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 probiotic bacteria on lipopolysaccharide (LPS)‐stimulated phorbol 12‐myristate 13‐acetate (PMA)‐differentiated THP‐1 cells. Methods and Results After culturing the bacterium, the crude cell‐free supernatant was fractionated on the basis of molecular weights using ultrafiltration. Also, a heat‐killed and sonicated fraction was obtained from the biomass of the bacterial culture. All fractions were used to measure their anti‐inflammatory effects on PMA‐differentiated THP‐1 cells following LPS stimulation by quantifying various cellular markers of inflammation. The results demonstrated that various L. reuteri PTCC 1655‐derived fractions, especially the >100 kDa supernatant fraction decreased some of the inflammatory cytokines and mediators, including tumour necrosis factor‐α, interleukin‐1, nitric oxide, cyclooxygenase‐2, matrix metalloproteinase‐9 and interleukin‐6, which are critical for the pathogenesis of some inflammatory diseases. Conclusion It is concluded that the L. reuteri PTCC 1655‐derived high molecular weight fractions significantly reduce inflammation and therefore could be appropriate candidates for future medical studies. Significance and Impact of the Study Providing new insights about the significance of L. reuteri PTCC 1655‐derived extracts and their potential to modulate inflammation.