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Microbially induced carbonate precipitation via methanogenesis pathway by a microbial consortium enriched from activated anaerobic sludge
Author(s) -
Su F.,
Yang Y.Y.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14930
Subject(s) - methanogenesis , microbial consortium , environmental chemistry , chemistry , pulp and paper industry , bacteria , biology , microorganism , methane , organic chemistry , genetics , engineering
Aims Various applications of microbially induced carbonate precipitation (MICP) has been proposed. However, most studies use cultured pure strains to obtain MICP, ignoring advantages of microbial consortia. The aims of this study were to: (i) test the feasibility of a microbial consortium to produce MICP; (ii) identify functional micro‐organisms and their relationship; (iii) explain the MICP mechanism; (iv) propose a way of applying the MICP technique to soil media. Methods and Results Anaerobic sludge was used as the source of the microbial consortium. A laboratory anaerobic sequencing batch reactor and beaker were used to perform precipitation experiment. The microbial consortium produced MICP with an efficiency of 96·6%. XRD and SEM analysis showed that the precipitation composed of different‐size calcite crystals. According to high‐throughput 16S rRNA gene sequencing, the functional micro‐organisms included acetogenic bacteria, acetate‐oxidizing bacteria and archaea Methanosaeta and Methanobacterium beijingense . The methanogenesis acetate degradation provides dissolved inorganic carbon and increases pH for MICP. A series of reactions catalysed by many enzymes and cofactors of methanogens and acetate‐oxidizers are involved in the acetate degradation. Conclusion This work demonstrates the feasibility of using the microbial consortium to achieve MICP from an experimental and theoretical perspective. Significance and Impact of the Study A method of applying the microbial‐consortium MICP to soil media is proposed. It has the advantages of low cost, low environmental impact, treatment uniformity and less limitations from natural soils. This method could be used to improve mechanical properties, plug pores and fix harmful elements of soil media, etc.