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Positioning Bacillus subtilis as terpenoid cell factory
Author(s) -
Pramastya H.,
Song Y.,
Elfahmi E.Y.,
Sukrasno S.,
Quax W.J.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14904
Subject(s) - bacillus subtilis , terpenoid , metabolic engineering , isoprene , terpene , biochemistry , biosynthesis , synthetic biology , metabolic pathway , biology , chemistry , biochemical engineering , computational biology , enzyme , microbiology and biotechnology , organic chemistry , bacteria , engineering , genetics , copolymer , polymer
Summary Increasing demands for bioactive compounds have motivated researchers to employ micro‐organisms to produce complex natural products. Currently, Bacillus subtilis has been attracting lots of attention to be developed into terpenoids cell factories due to its generally recognized safe status and high isoprene precursor biosynthesis capacity by endogenous methylerythritol phosphate (MEP) pathway. In this review, we describe the up‐to‐date knowledge of each enzyme in MEP pathway and the subsequent steps of isomerization and condensation of C5 isoprene precursors. In addition, several representative terpene synthases expressed in B. subtilis and the engineering steps to improve corresponding terpenoids production are systematically discussed. Furthermore, the current available genetic tools are mentioned as along with promising strategies to improve terpenoids in B. subtilis , hoping to inspire future directions in metabolic engineering of B. subtilis for further terpenoid cell factory development.