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Antifungal and anti‐biofilm effects of 6‐shogaol against Candida auris
Author(s) -
Kim H.R.,
Eom Y.B.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14870
Subject(s) - biofilm , candida auris , microbiology and biotechnology , antifungal , in vitro , chemistry , biology , bacteria , biochemistry , genetics
Aims This study aimed to assess the antifungal and anti‐biofilm effects of 6‐shogaol against Candida auris using in vitro phenotypic and genotypic analyses. Methods and Results Our results showed that 6‐shogaol exhibited antifungal as well as anti‐biofilm activity by inhibiting biofilm formation and eradicating the preformed biofilms of C. auris . The rate and extent of antifungal activity were further confirmed by a time‐kill assay. The XTT reduction assay confirmed that 6‐shogaol decreased cellular metabolic activity in the biofilm. The effect of 6‐shogaol on established C. auris biofilms was visualized by confocal laser scanning microscopy. Also, this study demonstrated that 6‐shogaol reduced the levels of aspartyl proteinases and downregulated the expression of the efflux pump‐related CDR1 gene in C. auris . Conclusions The data indicated that 6‐shogaol extracted from ginger had antifungal and anti‐biofilm effects on C. auris . Significance and Impact of the Study This study demonstrated the value of the plant‐derived 6‐shogaol as a promising and potent bioactive compound. The mode of action of this compound against C. auris biofilm was also proposed.