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Prevalence, molecular typing and antimicrobial susceptibility of Campylobacter spp. isolates in northern Spain
Author(s) -
Nafarrate I.,
Lasagabaster A.,
Sevillano E.,
Mateo E.
Publication year - 2021
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14842
Subject(s) - campylobacter , biology , campylobacteriosis , campylobacter coli , genotype , pulsed field gel electrophoresis , microbiology and biotechnology , tetracycline , campylobacter jejuni , campylobacter fetus , ciprofloxacin , antibiotic resistance , molecular epidemiology , nalidixic acid , erythromycin , antimicrobial , veterinary medicine , antibiotics , medicine , bacteria , genetics , gene
Aim To analyse the prevalence, genetic diversity and antimicrobial susceptibility of Campylobacter spp. in northern Spain. Methods and results Campylobacter was isolated from 139 samples of broiler meat and faecal dropping of broiler and swine with a prevalence of 35·4, 62 and 42·8%, respectively. Campylobacter jejuni (n = 55) and Campylobacter coli ( n = 31) were identified by multiplex‐PCR in meat, faeces and human clinical samples while Campylobacter fetus (n = 3) was exclusively detected in the latter. Fingerprinting by fla A‐RFLP and PFGE revealed 68 different genotypes from the 89 isolates with a Biodiversity Simpson’s index of 0·98. The 86·5% of the isolates were resistant to ciprofloxacin, 85·4% to tetracycline and 49·4% to erythromycin; only three genotypes were susceptible to the three antimicrobial drugs. Multidrug resistance was detected in the 40·7% of the isolates. Conclusions Campylobacter remains prevalent in northern Spain with a high biodiversity degree. About 93·3% of the isolates were resistant to one or more drugs. Significance and Impact of the Study Although different measures are taken to control Campylobacter , the detection of isolates resistant to the drugs used in the treatment of campylobacteriosis is still high, including different species and genotypes. This evidences the need of additional strategies against this pathogen.