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Immunosensor‐based rapid quantitative detection of Newcastle disease virus antibodies using innovative gold immunochromatographic assay
Author(s) -
Yang F.,
Li Y.,
Jin X.,
Xu Q.,
Cheng F.,
Wang X.
Publication year - 2020
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14688
Subject(s) - newcastle disease , antibody , detection limit , gold standard (test) , virus , virology , hemagglutination , biology , chromatography , chemistry , medicine , immunology
Aims A novel quantitative method for rapid Newcastle disease virus (NDV) antibody detection was developed based on an innovative gold immunochromatographic assay with a quantitative immunosensor. Methods and Results NDV antibody‐detecting test strips containing a two‐reaction system and double‐test lines (T1, T2) were prepared. The test results were judged according to the signal ratio between the test and control lines as measured by the quantitative immunosensor. The minimum detection limit of the test strips for NDV antibodies was 2 2 titres. In addition, the assay was highly specific because only NDV antibodies produced visible test lines on the strip. The clinical application of the strips was tested by detecting NDV antibodies in 506 serum samples collected from chickens. The results showed a coincidence of 92·49% with those of the haemagglutination inhibition assay. Conclusions The strips were successfully prepared and showed high specificity towards NDV, sensitivity and stability. Significance and Impact of the Study This study describes a new method for detection of NDV antibody and provides a reference basis for rapid and quantitative monitoring of NDV antibodies. This new method overcomes the limitation of the existing colloidal gold immunochromatography, which only produces qualitative or semi‐quantitative results.