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Determination of gastrointestinal tract colonization sites from feedlot cattle transiently shedding or super‐shedding Escherichia coli O157:H7 at harvest
Author(s) -
Wells J.E.,
Berry E.D.,
Kim M.,
Bono J.L.,
Oliver W.T.,
Kalchayanand N.,
Wang R.,
Freetly H.C.,
Means W.J.
Publication year - 2020
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14684
Subject(s) - biology , abomasum , colonization , feces , veterinary medicine , rumen , gastrointestinal tract , microbiology and biotechnology , zoology , escherichia coli , eimeria , cecum , pathogen , ecology , food science , medicine , fermentation , gene , biochemistry
Aims The objective of the study was to determine levels of Escherichia coli O157:H7 colonization in the gastrointestinal tract (GIT) of naturally shedding cattle shedding the pathogen at low‐ or super‐shedder levels. Methods and Results Over 2 years, feedlot cattle were sampled multiple times for faecal shedding of E. coli O157:H7. Just prior to harvest (1–2 days), animals that were super‐shedders (≥10 4 CFU per gram of faeces) were specifically identified, and based on the longer term screening data, pen cohorts that were low‐shedders (years 1 and 2) or chronic‐shedders (year 1) were also identified. At harvest, samples were collected from throughout the GIT, including the rectoanal junction (RAJ) for enumeration and enrichment of E. coli O157:H7. The mouth samples exhibited the greatest prevalence for the pathogen, and the abomasum and rumen exhibited the lowest prevalence ( P < 0·05). Super‐shedders had significantly greater prevalence for all GIT locations except the mouth and abomasum compared to low‐shedders, but the super‐shedders were the only animals with positive abomasum samples. Samples from the super‐shedders were enumerable for most GIT locations, and the rectum and RAJ locations were the only locations that were significantly greater than other locations ( P < 0·05). Conclusions Across all animals naturally exposed to E. coli O157:H7, the risk of ingestion is high, but rumen and abomasum are potential barriers to passage. In super‐shedders, the passage through the GIT was greater, allowing colonization in the rectum and at the RAJ. Significance and Impact of the Study Escherichia coli O157:H7 low‐shedding cattle had lower pathogen levels throughout the GIT, indicating intrinsic GIT factors to these cattle may reduce pathogen passage through the GIT, including the abomasum, and minimize risk of RAJ colonization.