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A compound isolated from Alpinia officinarum Hance. inhibits swarming motility of Pseudomonas aeruginosa and down regulates virulence genes
Author(s) -
Lakshmanan D.,
Harikrishnan A.,
Jyoti K.,
Idul Ali M.,
Jeevaratnam K.
Publication year - 2020
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14563
Subject(s) - virulence , swarming motility , pseudomonas aeruginosa , swarming (honey bee) , microbiology and biotechnology , biology , gene , alpinia , pseudomonadales , pseudomonadaceae , pseudomonas , motility , quorum sensing , bacteria , genetics , botany
Aim The study was aimed at purifying the active principle from Alpinia officinarum rhizomes responsible for inhibition of swarming motility of Pseudomonas aeruginosa and analysing the mechanism of action. Methods and Results The active compound from methanol extract of A. officinarum was purified by silica gel column chromatography followed by elution from Amberlite resin. The compound 1‐(3,5‐dihydroxyphenyl)‐2‐(methylamino)ethan‐1‐one, inhibited swarming motility at 12·5 µg ml −1 . This inhibition was independent of rhamnolipid production. Real‐time PCR analysis showed significant down‐regulation of virulence‐associated genes including T3SS exo S, exo T and flagella master regulator fle Q. Conclusions The compound from A. officinarum inhibited swarming motility and significantly down‐regulated the expression of type III secretory system effector genes exo S and exo T and flagellar master regulator fle Q genes. Significance and Impact of the Study The study identifies a potent swarming inhibitory compound from the common medicinal plant A. officinarum and reinstates the potential of plant‐derived compounds in tackling virulence properties of pathogenic bacteria.