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Antimicrobial resistance of pathogenic Aeromonas spp. isolated from marketed Pacific abalone ( Haliotis discus hannai ) in Korea
Author(s) -
Wickramanayake M.V.K.S.,
Dahanayake P.S.,
Hossain Sabrina,
Heo GangJoon
Publication year - 2020
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14485
Subject(s) - aeromonas , microbiology and biotechnology , abalone , biology , aeromonas salmonicida , virulence , aeromonas veronii , aeromonas hydrophila , nalidixic acid , ampicillin , antibiotic resistance , antimicrobial , oxytetracycline , antibiotics , bacteria , fishery , gene , biochemistry , genetics
Aims The object of this study was to identify potential health concerns of the Aeromons spp. isolated from marketed Pacific abalone ( Haliotis discus hannai ) with respect to their virulence and antimicrobial resistance patterns. Methods and Results We identified 29 strains of aeromonads consisting of five species; Aeromonas hydrophila ( n = 9), Aeromonas enteropelogenes ( n = 14), Aeromonas veronii ( n = 3), Aeromonas salmonicida ( n = 2) and Aeromonas sobria ( n = 1), by employing series of biochemical tests and gene sequencing. In the phenotypic virulence assays, all isolates showed gelatinase and caseinase activities, while lipase formation (69%), phospholipase production (90%), DNase formation (82%), slime production (49%) and haemolysis activity ( α = 18% and β = 82%) were also detected among isolates. Prevalence of virulence genes; aerA (100%), fla (66%), ahyB (73%), act (52%), alt (42%), ast (35%), ser (52%), gcat (69%), ascV (43%), hlyA (83%), lip (52%) and exu (59%) were detected by PCR assays. In disc diffusion test, 100% resistance was detected against ampicillin while cephalothin, rifampicin, oxytetracycline, colistine sulphate, nalidixic acid and piperaciliin were resisted by 86, 73, 42, 35, 28, 20 and 20% of the isolates respectively. Thirteen (45%) of the isolates showed multiple antimicrobial resistance (MAR) indices ≥ 0·2. Conclusions Our findings suggest that the potential health risk posed by the abalone‐borne Aeromonas spp. should not be underestimated. Significance and Impact of the Study This is the first time to evaluate possible public health risks upon consumption of abalone harbored Aeromonas spp. and also to isolate potential pathogenic and multidrug‐resistant Aeromonas spp . from Pacific abalone in Korea.