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Unique secondary metabolites of a Streptomyces strain isolated from extreme salty wetland show antioxidant and antibacterial activities
Author(s) -
Riahi K.,
Hosni K.,
Raies A.,
Oliveira R.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14428
Subject(s) - extreme environment , strain (injury) , streptomyces , antioxidant , microbiology and biotechnology , biology , antibacterial activity , chemistry , biochemistry , bacteria , genetics , anatomy
Aims To identify and assess the biological activities of the crude extract of a Streptomyces isolate from a salty wetland, an extreme environment likely to induce secondary metabolism of micro‐organisms. Methods and Results The crude extract from the isolate Streptomyces lanatus strain AR2 displayed antibacterial activity against Gram‐positive bacteria (MICs ranging from 5 to 50 μg ml −1 ) and antioxidant activity as revealed in DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) assay (IC 50 of 0·74 mg ml −1 ), ferric reducing antioxidant power (IC 50 of 1·12 mg ml −1 ) and metal‐chelating power (IC 50 of 1·84 mg ml −1 ) assays. Accordingly, the extract attenuated the H 2 O 2 ‐induced oxidative stress in the eukaryotic cell model Saccharomyces cerevisiae , assessed by flow cytometry . The profiling of secondary metabolites by HPLC‐PDA‐ESI‐MS/MS revealed the presence of 17 compounds, some of which reported in Streptomyces for the first time to the best of our knowledge: genistein‐7‐ O ‐glucuronide, naringenin‐7‐ O ‐rutinoside and resveratrol. Conclusions Streptomyces lanatus AR2 produced unique polyketides and phenolic compounds with noticeable bioactivities, allowing adaptation to the extreme environment. Significance and Impact of the Study Sabkhat Seijoumi salty wetland represents a potential niche for Streptomyces yielding useful natural products for biotechnological, pharmaceutical and medical applications.