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Evaluating the antibacterial and antibiofilm potential of sulphated polysaccharides extracted from green algae Chlamydomonas reinhardtii
Author(s) -
Vishwakarma J.,
Vavilala S.L.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14364
Subject(s) - biofilm , microbiology and biotechnology , extracellular polymeric substance , chlamydomonas reinhardtii , bacillus subtilis , polysaccharide , bacteria , minimum inhibitory concentration , agar , antibacterial activity , escherichia coli , chemistry , biology , antibiotics , biochemistry , genetics , mutant , gene
Aim of the study In this study, antibacterial and antibiofilm potential of sulphated polysaccharides (SPs) extracted from Chlamydomonas reinhardtii (Cr) was evaluated against Neisseria mucosa , Escherichia coli , Streptococcus sp. and Bacillus subtilis . Methods and Results Antibacterial potential of Cr‐SPs was evaluated by agar‐cup diffusion, time‐kill and colony‐forming ability (CFU), minimum inhibitory and bactericidal concentration assays. Antibiofilm potential was evaluated by biofilm inhibition, eradication, extracellular‐DNA, metabolic activity and microscopy assays. Cr‐SPs at 0·5 mg ml −1 showed 34·52, 48·6, 66·1 and 55·6% reduced CFU in B. subtilis , Streptococcus , N. mucosa and E. coli respectively. Minimum inhibitory concentration of Cr‐SPs was as low as 480 μg ml −1 for Streptococcus , N. mucosa and 420 μ g ml −1 for B. subtilis and E. coli . At 1 mg ml −1 , Cr‐SPs showed 50% biofilm inhibition, whereas 4–8 mg ml −1 showed 100% inhibition. Cr‐SPs also effectively dissolved preformed biofilms. Dose‐dependent reduction in extracellular DNA revealed that Cr‐SPs interacts with the extra polymeric substance of the biofilm and destroys them. Light microscopy reconfirmed the above results. Conclusion Cr‐SPs not only inhibited biofilm formation but also effectively dissolved preformed‐biofilms. Significance and Impact of the Study The current study showed the promising potential of Cr‐SPs as antibiofilm agents. Further validation will help in developing Cr‐SPs as natural antibiotics against biofilm‐causing bacteria.