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Macrocystis pyrifera source of nutrients for the production of carotenoids by a marine yeast Rhodotorula mucilaginosa
Author(s) -
Leyton A.,
Flores L.,
MäkiArvela P.,
Lienqueo M.E.,
Shene C.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14362
Subject(s) - macrocystis pyrifera , carotenoid , astaxanthin , food science , yeast extract , yeast , nutrient , biology , botany , algae , chemistry , biochemistry , fermentation , ecology
Aims To evaluate an aqueous extract of Macrocystis pyrifera as a nutrient source for the production of carotenoids by a marine Rhodotorula mucilaginosa isolated from seaweed samples. Materials and Results The effect of different culture conditions on the concentration of biomass and total pigments was evaluated using a Box–Behnken experimental design. The seaweed extract contained 15% w w −1 of protein and 20% w w −1 of carbohydrate; the main sugar in this fraction was trehalose (78%). The culture conditions that maximize the total pigment concentration (1·84 ± 0·03 mg l −1 ) were initial pH equal to 7, yeast extract as nitrogen source at a concentration of 4 g l −1 , seaweed extract concentration at 25% v v −1 , incubation performed at 25°C and 150 rev min −1 during 6 days. Under optimal growth conditions, three carotenoids were identified among the pigments produced by R. mucilaginosa , lycopene (38·4 ± 9·4%), β‐carotene (21·8 ± 1·5%) and astaxanthin (1·8 ± 0·3%). Conclusions Carotenoids of commercial interest (lycopene, β‐carotene and astaxanthin) can be produced using a marine R. mucilaginosa cultivated with an aqueous extract of M. pyrifera as nutrient source. The total pigment concentration in the culture ranged between 0·82 and 1·84 mg l −1 , and was significantly affected by the concentration of the seaweed extract, and yeast extract. Significance and Impact of the Study This work demonstrates that M. pyrifera can be used as a nutrient source for the production of carotenoids by the marine yeast.