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Effects of Lactococcus lactis MG1363 producing fusion proteins of bovine lactoferricin–lactoferrampin on growth, intestinal morphology and immune function in weaned piglet
Author(s) -
Song L.,
Qiao X.,
Zhao D.,
Xie W.,
Bukhari S.M.,
Meng Q.,
Wang L.,
Cui W.,
Jiang Y.,
Zhou H.,
Li Y.,
Xu Y.,
Tang L.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14339
Subject(s) - biology , immune system , lactococcus lactis , microbiology and biotechnology , morphology (biology) , bacteria , immunology , lactic acid , zoology , genetics
Aims We developed a strategy for localized delivery of the LFCA (lactoferricinlactoferrampin), which is actively synthesized in situ by Lactococcus lactis (pAMJ399‐LFCA/LLMG1363), and explored the possibility of using pAMJ399‐LFCA/LLMG1363 as an alternative additive diet to antibiotics. Methods and Results The antimicrobial activities of the LFCA derived from pAMJ399‐LFCA/LLMG1363 were tested in vitro . The results showed that LFCA had an inhibitory effect on Staphylococcus aureus , Escherichia coli and Salmonella enteritidis . Then, the pAMJ399‐LFCA/LLMG1363 was used as an additive diet for piglets. Our data demonstrated that oral administration of pAMJ399‐LFCA/LLMG1363 significantly improved the average daily gain, feed‐to‐gain ratio, intestinal mucosal integrity and decreased the serum endotoxin and d ‐lactic acid levels. The mRNA expression levels of intestinal tight junction proteins (including occludin, Claudin‐1 and ZO‐1) were significantly upregulated by pAMJ399‐LFCA/LLMG1363 administration. The serum immunoglobulin G (IgG) levels, intestinal secretory immunoglobulin A (sIgA) levels, IL‐2, IL‐10 and TGF‐β levels were significantly increased by pAMJ399‐LFCA/LLMG1363. Furthermore, our data revealed that oral administration of pAMJ399‐LFCA/LLMG1363 significantly increased the number of general Lactobacillus , and decreased the total viable E. coli counts in the ileum and cecum contents. Conclusions We developed a novel pAMJ399‐LFCA/LLMG1363 secreting LFCA, which had probiotic effects on the growth performance, intestinal morphology, intestinal barrier function and immunological indices of weaned piglets. Significance and Impact of the Study pAMJ399‐LFCA/LLMG1363, with probiotic effects on the health of weaned piglets, may be a promising feed additive for weaned piglets.