Premium
Probiotic effect of Bifidobacterium longum 5 1A and Weissella paramesenteroides WpK4 on gerbils infected with Giardia lamblia
Author(s) -
Fonseca J.F.,
Alvim L.B.,
Nunes Á.C.,
Oliveira F.M.S.,
Amaral R.S.,
Caliari M.V.,
Nicoli J.R.,
Neumann E.,
Gomes M.A.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14338
Subject(s) - bifidobacterium longum , probiotic , biology , microbiology and biotechnology , giardia lamblia , bifidobacterium , mucus , giardia , bacteria , duodenum , gastroenterology , medicine , lactobacillus , ecology , genetics
Aims The objective of this study was to assess the probiotic potential of genuine strains of Bifidobacterium longum 5 1A and Weissella paramesenteroides WpK4, in experimental giardiasis. Methods and Results The bacteria were administered orally to gerbils ( Meriones unguiculatus ) 10 days before oral infection with trophozoites of Giardia lamblia . After 7 days of infection, the animals were euthanized and portions of the duodenum were processed for histopathologic, histochemical and morphometric assessment. The height of the intestinal crypts and crypt/villi ratio were higher in infected groups ( P < 0·05) than in noninfected groups. The area of mucus production was higher ( P < 0·05) in infected animals pretreated with B. longum 5 1A than in other groups. The parasitic load of the animals that received both bacteria decreased significantly ( P < 0·05) compared to the ones of the control group. Conclusions Our results suggest a probiotic function of B. longum 5 1A and W. paramesenteroides WpK4 and may result in their use as a prophylactic and therapeutic alternative for promoting human and animal health. Significance and Impact of the Study Bifidobacterium longum 5 1A and W. paramesenteroides WpK4 may constitute prophylactic alternatives, reversing the emergence of side effects and resistance observed in the conventional treatment of giardiasis.