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Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil actinobacteria
Author(s) -
Thilagam R.,
Hemalatha N.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14259
Subject(s) - colletotrichum capsici , biology , actinobacteria , rhizosphere , rhizobacteria , horticulture , beneficial organism , biofertilizer , streptomyces , germination , phytophthora capsici , pathogen , botany , bacteria , microorganism , fungicide , microbiology and biotechnology , 16s ribosomal rna , pepper , genetics
Aim The aim of this study was to screen potential plant growth promoting rhizobacterial ( PGPR ) actinobacterial isolate with effective inhibition against anthracnose causing fungal pathogen Colletotrichum capsici . Methods and Results In this study, actinobacterias were isolated from rhizosphere soil using dilution plate method and tested for antagonistic potential against pathogenic fungi C. capsici . In primary and secondary screening tests, the actinobacterial isolate BS ‐26 displayed high antagonistic activity against the fungal pathogen. Isolate BS ‐26 was identified as Streptomyces violaceoruber based on 16S rDNA sequencing. Furthermore, indole acetic acid production, phosphate solubilization and ammonia production have been confirmed in the S. violaceoruber that suggest their potential to be used as PGPR bacteria. A green house experiment showed that application of S. violaceoruber fermentation broth reduced the incidence of the chilli anthracnose and promoted the growth of chilli seedlings with a significant increase in germination %, total plant height, fresh weight and chlorophyll content when compared to controls. Conclusion Streptomyces violaceoruber can be applied as a biofertilizer and biocontrol agent for growing chillies against the attack of fungal pathogen C. capsici . Significance of Impact of the Study The damage caused by anthracnose disease is an issue of concern, affecting negatively the economy involved in chilli cultivation. As chemical methods of control have serious disadvantages, biocontrol approach using beneficial ( PGPR ) micro‐organisms shall be a better alternative to control crop diseases.