Premium
Production of prebiotic xylooligosaccharide from aqueous ammonia‐pretreated rice straw by β‐xylosidase of Weissella cibaria
Author(s) -
Le B.,
Yang S.H.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14255
Subject(s) - prebiotic , xylan , chemistry , food science , straw , hydrolysis , xylanase , cellulose , lignin , biochemistry , organic chemistry , enzyme , inorganic chemistry
Aims This study focuses on the development of a new strategy xylooligosaccharide ( XOS ) production from aqueous ammonia‐pretreated rice straw (A‐ PRS ), followed by β‐xylosidase hydrolysis produced by the newly identified strain Weissella cibaria FB 069. Methods and Results We report a higher efficiency of A‐ PRS , including the removal of lignin and increase in cellulose and xylan content, compared to that of the alkali and stream explosion methods. Using the ammonia pretreatment method, rice straw was used to obtain 32·4% xylan. The crude xylanase from W. cibaria was used to hydrolyse A‐ PRS over different hydrolysis times. The highest XOS yield (131 mg XOS per gram rice straw) was observed after 10 h. XOS produced from the PRS was tested on stimulation effect on Bifidobacterium and Lactobacillus . Conclusion The possibility of XOS production from PRS using β‐xylosidase with strong prebiotic properties. Significance and Impact of the Study We investigated the new strain for signification production of XOS . The two‐stage process here described could help to further explore the optimization conditions for prebiotic production. Additionally, the stimulation effect of XOS from alternative source has a promising prospect in functional food.