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TRAIL ‐expressing recombinant Lactococcus lactis induces apoptosis in human colon adenocarcinoma SW 480 and HCT 116 cells
Author(s) -
Bohlul E.,
Hasanlou F.,
Taromchi A.H.,
Nadri S.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14237
Subject(s) - lactococcus lactis , apoptosis , recombinant dna , biology , cell culture , microbiology and biotechnology , programmed cell death , cell , viability assay , gene , biochemistry , bacteria , lactic acid , genetics
Aims We investigated the ability of Lactococcus lactis, a species generally regarded as safe, to express tumour necrosis factor‐related apoptosis‐inducing ligand ( TRAIL ) protein. The expressed protein was either cell wall anchored or secreted, and it was assessed whether this could induce apoptosis in human colon adenocarcinoma cell lines SW 480 and HCT 116. Methods and Results Constructs were designed to produce either secreted or cell wall‐anchored forms of human TRAIL cloned into pNZ 7021 expression vector. The expression by L. lactis was confirmed by Western blotting and immunofluorescence. Induction of cell death was evaluated by coculturing transformants producing either form of TRAIL protein with the two cell lines followed by MTT assay. Gene expression of apoptosis genes, Bax and Bcl2 , was assessed by qPCR . The viability of SW 480 and HCT 116 cells treated with recombinant L. lactis was significantly reduced. A significant change was observed in the ratio of Bax/Bcl2 expression in HCT 116 cells only following treatment with the supernatant of recombinant L. lactis containing secreted TRAIL. Conclusion Recombinant L. lactis producing TRAIL protein can induce apoptosis in human colon adenocarcinoma cell lines SW 480 and HCT 116. Significance and Impact of the Study The use of recombinant probiotics that produce anticancer compounds is a promising option for combating cancer cells.