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Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp‐115
Author(s) -
Zhou Y.,
Wang J.Q.,
Hu C.H.,
Ren L.Q.,
Wang D.C.,
Ye B.C.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14229
Subject(s) - lactobacillus plantarum , maltodextrin , microbiology and biotechnology , food science , lactobacillus , chemistry , biology , bacteria , lactic acid , chromatography , fermentation , spray drying , genetics
Aims To identify the mechanism in which way maltodextrin enhance bile tolerance in Lactobacillus plantarum Lp‐115. Methods and Results Based on determining the OD 600 value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp‐115. The OD 600 value of L. plantarum Lp‐115 cultured in MRSB broth with maltodextrin was three times higher than the control value. After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%. Conclusions In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene ( pva3 ) significantly. Significance and Impact of the Study This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance. This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.