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Construction of a novel DNA vaccine candidate targeting F gene of genotype VII Newcastle disease virus and chicken IL ‐18 delivered by Salmonella
Author(s) -
Gao X.,
Xu K.,
Yang G.,
Shi C.,
Huang H.,
Wang J.,
Yang W.,
Liu J.,
Liu Q.,
Kang Y.,
Jiang Y.,
Wang C.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14228
Subject(s) - newcastle disease , salmonella , virus , dna vaccination , genotype , gene , biology , virology , microbiology and biotechnology , bacteria , genetics , recombinant dna
Aims Genotype VII Newcastle disease ( ND ) is one of the most epidemic and serious infectious diseases in the poultry industry. A novel vaccine targeting VII Newcastle disease virus ( NDV ) is still proving elusive. Methods and Results In this study, we constructed regulated delayed lysis Salmonella strains expressing either a fusion protein (F) alone under an eukaryotic CMV promoter or together with chicken IL ‐18 (ch IL ‐18) as a molecular adjuvant under prokaryotic P trc promoter, named pYL 1 and pYL 23 respectively. Oral immunization with recombinant strains induced NDV ‐specific serum IgG antibodies in both pYL 1‐ and pYL 23‐immunized chickens. The presence of ch IL ‐18 significantly increased lymphocyte proliferation in immunized chickens, as well as the percentages of CD 3 + CD 4 + and CD 3 + CD 8 + T cells in serum, even if a statistically significant difference did not exist. After a virulent challenge, pYL 23 immunization provided about 80% protection at day 10 postinfection, compared with 60% of protection offered by pYL1 immunization and 100% protection in the inactivated vaccine group, indicating the enhanced immune response provided by ch IL ‐18, which was also confirmed by histochemical analysis. Conclusions Recombinant lysis Salmonella ‐vectored DNA vaccine could provide us a novel potential option for controlling NDV infection. Significance and Impact of the Study This study took use of a regulated delayed lysis Salmonella vector for the design of an orally administrated vaccine against NDV .