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Antifungal mechanisms of α‐terpineol and terpene‐4‐alcohol as the critical components of Melaleuca alternifolia oil in the inhibition of rot disease caused by Aspergillus ochraceus in postharvest grapes
Author(s) -
Kong Q.,
Zhang L.,
An P.,
Qi J.,
Yu X.,
Lu J.,
Ren X.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14193
Subject(s) - aspergillus ochraceus , postharvest , terpineol , preservative , melaleuca alternifolia , terpene , chemistry , food science , biology , antimicrobial , botany , essential oil , microbiology and biotechnology , biochemistry , organic chemistry , mycotoxin , ochratoxin a , tea tree oil
Aims As a natural antimicrobial agent, Melaleuca alternifolia oil (MAO) is generally recognized to be safe and effective in the inhibition of phytopathogenic fungi. Due to lack of comprehensive studies on MAO for controlling postharvest Aspergillus, we investigated the preservative mechanism of MAO and its components against Aspergillus ochraceus in postharvest grapes to evaluate their potential effectiveness as fruit preservatives. Methods and Results In our study, the compositions in MAO were analysed by gas chromatography–mass spectrometry. The inhibitory effects of MAO and its main constituents against A. ochraceus were compared by scanning electron microscopy and transmission electron microscopy observation, and metabolic analysis. Two components of MAO, α‐terpineol and terpene‐4‐alcohol, showed higher antifungal effects than MAO, of which α‐terpineol caused the worst leakage of cytoplasm and most serious hyphae distortions and spore disruptions. The downregulation of metabolic pathways of A. ochraceus was strongest with α‐terpineol. The best inhibitory efficacy against A. ochraceus in grapes also occurred with α‐terpineol. 3‐Carene showed little inhibitory effect. Conclusions These results demonstrate that not all components in MAO possess antimicrobial effects, and α‐terpineol is the main contributor of MAO's A. ochraceus inhibition effect. Significance and Impact of the Study α‐Terpineol may be used as an alternative natural preservative for the postharvest storage of grapes and other fruits.