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Stabilization and controlled release of micro‐encapsulated hydrogen peroxide for wound treatment applications
Author(s) -
DoganGuner E.M.,
Mohamed H.,
Orbey N.,
Goodyear N.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14177
Subject(s) - self healing hydrogels , hydrogen peroxide , antimicrobial , enterococcus faecalis , controlled release , chemistry , staphylococcus aureus , nuclear chemistry , microbiology and biotechnology , sorption , biofilm , materials science , nanotechnology , polymer chemistry , bacteria , biochemistry , biology , organic chemistry , adsorption , genetics
Abstract Aims Hydrogen peroxide (H 2 O 2 ) disinfection applications are limited by its rapid inactivation. The aims of this study were to (i) micro‐encapsulate H 2 O 2 in silica hydrogels to obtain controlled release up to 72 h, (ii) test hydrogel antimicrobial activity against four common pathogens and (iii) assess H 2 O 2 release kinetics and antimicrobial activity in 35–65% relative humidity and 37°C to approximate bandaged wound conditions. Methods and Results Hydrogels were characterized using scanning electron microscopy, nitrogen sorption porosimetry, Brunauer Emmet Teller analysis and the Barret–Joyner–Halenda method. Hydrogels formed at lower pH demonstrated increased surface area and decreased pore size, resulting in H 2 O 2 release lasting 72 h. Using agar well diffusion for antimicrobial activity, statistically significant zones of inhibition (as compared to controls) were seen for Escherichia coli , Staphylococcus aureus, Pseudomona aeruginiosa and Vancomycin‐resistant Enterococcus faecalis . Activity remained for hydrogels aged for 72 h in humid, 37°C conditions. Conclusions Hydrogels can be synthesized to provide a continuous, controlled release of H 2 O 2 for up to 72 h. Significance and Impact of the Study Stable, controlled‐release H 2 O 2 hydrogels have potential applications for wound treatment and disinfection of medical equipment, through bonding to bandages or materials such as catheter lumens.