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Impact of probiotic Lactobacillus sp. on autochthonous lactobacilli in weaned piglets
Author(s) -
Zhao X.,
Wang W.,
Blaine A.,
Kane S.T.,
Zijlstra R.T.,
Gänzle M.G.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14119
Subject(s) - lactobacillus fermentum , lactobacillus reuteri , probiotic , lactobacillus casei , lactobacillus salivarius , biology , lactobacillus , microbiology and biotechnology , feces , food science , fermentation , bacteria , lactic acid , genetics , lactobacillus plantarum
Aims This study aimed to determine whether host‐adapted lactobacilli exhibit superior survival during intestinal transit relative to nomadic and free‐living organisms, and to characterize the impact of probiotic lactobacilli on autochthonous lactobacilli. Methods and Results Mixed cultures of Lactobacillus casei K9‐1 and Lactobacillus fermentum K9‐2, or reutericyclin producing Lactobacillus reuteri and its isogenic mutant were fed to piglets as freeze‐dried culture, or as part of fermented feed. Lactobacilli in digesta and faecal samples were quantified by strain‐specific quantitative PCR ( qPCR ), high‐resolution‐melting curve qPCR , and high‐throughput sequencing of 16S rRNA gene sequence tags. The abundance of the host adapted L. reuteri in digesta and faeces was higher ( P < 0·05) when compared to L. casei or L. fermentum . Feed fermentation or chemical acidification of feed reduced ( P < 0·05) cell counts of Lactobacillus salivarius in colonic digesta. The reutericyclin producing L. reuteri TMW 1.656 transiently reduced ( P < 0·05) the faecal abundance of lactobacilli. However, the overall impact of probiotic intervention on autochthonous lactobacilli was minor. Conclusions The vertebrate host‐adapted L. reuteri survives better during intestinal transit of piglets compared to L. casei and L. fermentum . Significance and Impact of the Study Ecology and lifestyle of Lactobacillus strains may be suitable criteria for selection of probiotic strains for use in swine production.