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Characterization of Tolypocladium cylindrosporum (Hypocreales, Ophiocordycipitaceae) isolates from Brazil and their efficacy against Aedes aegypti (Diptera, Culicidae)
Author(s) -
Montalva C.,
Silva J.J.,
Rocha L.F.N.,
Luz C.,
Humber R.A.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14093
Subject(s) - aedes aegypti , hypocreales , biology , chikungunya , aedes , larva , vector (molecular biology) , dengue fever , yellow fever , veterinary medicine , ecology , virology , virus , medicine , ascomycota , biochemistry , gene , recombinant dna
Aims To survey and to characterize entomopathogenic fungi as natural enemies of mosquitoes in Central Brazil. Methods and Results Tolypocladium cylindrosporum (Hypocreales, Ophiocordycipitaceae) was isolated for the first time in South America by using Aedes aegypti (Diptera, Culicidae) as sentinel larvae in a stagnant mosquito breeding site in a secondary tropical forest. Two isolates were morphologically and molecularly identified, and their activity tested against A. aegypti eggs, larvae and adults. Conclusions Findings emphasize the importance of T. cylindrosporum as a natural fungal antagonist of mosquitoes. Significance and Impact of the Study Both isolates affected larvae and adults but were less effective against eggs; both have potential for development as a mycoinsecticide especially against larvae of A. aegypti the main vector of dengue, chikungunya, Zika and urban yellow fever.