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Identification of common virulence factors present in enterotoxigenic Escherichia coli isolated from diarrhoeal patients in Kolkata, India
Author(s) -
Bhakat D.,
Debnath A.,
Naik R.,
Chowdhury G.,
Deb A.K.,
Mukhopadhyay A.K.,
Chatterjee N.S.
Publication year - 2019
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14090
Subject(s) - enterotoxigenic escherichia coli , virulence , enterotoxin , microbiology and biotechnology , biology , multiplex polymerase chain reaction , escherichia coli , virology , gene , polymerase chain reaction , genetics
Aims Development of an effective vaccine against enterotoxigenic Escherichia coli ( ETEC ) is largely dependent on the conscientious understanding of different virulence associated factors from diverse geographical areas. So, the objective of this study is to elucidate the distribution of enterotoxins, CF and NCVF in clinical ETEC strains isolated between 2008 and 2014 from two hospitals in Kolkata, India. Methods and Results Multiplex PCR method was used for detection of two enterotoxin genes, 11 common CF s and five common NCVF s. Among the 350 tested ETEC strains, 61% strains possessed est + elt genes, 25% est and 14% elt . Among 56% CF positive ETEC strains, CS 21 was the prevalent one (37%) followed by CS 6 (36%). NCVF genes were present in 59% of the ETEC strains; eatA was the most prevalent (65%) followed by etpA (51%). There were 29% strains negative for any CF s or NCVF s. Conclusions We conclude that a pattern exists between CS 6, eatA and toxins. We observed est with or without elt , CS 6 with or without CS 5 and with or without eatA were present in 24% of clinical ETEC strains (59/250) analysed. CS 21 has emerged as another predominant CF but it had diverse CF s and NCVF s. Significance and Impact of the Study Prevalence of ETEC virulence factors would help in tracking ETEC globally and suggests the need of a multivalent ETEC vaccine.