Immunogenicity and protective efficacy of orally administered recombinant  Lactobacillus plantarum  expressing  VP 2 protein against  IBDV  in chicken | Zendy

Maqsood I. | Zendy; Shi W. | Zendy; Wang L. | Zendy; Wang X. | Zendy; Han B. | Zendy; Zhao H. | Zendy; Nadeem A.M. | Zendy; Moshin B.S. | Zendy; Saima K. | Zendy; Jamal S.S. | Zendy; Din M.F. | Zendy; Xu Y. | Zendy; Tang L. | Zendy; Li Y. | Zendy

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Immunogenicity and protective efficacy of orally administered recombinant Lactobacillus plantarum expressing VP 2 protein against IBDV in chicken

Author(s) -

Maqsood I.,

Shi W.,

Wang L.,

Wang X.,

Han B.,

Zhao H.,

Nadeem A.M.,

Moshin B.S.,

Saima K.,

Jamal S.S.,

Din M.F.,

Xu Y.,

Tang L.,

Li Y.

Publication year - 2018

Publication title -

journal of applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.889

H-Index - 156

eISSN - 1365-2672

pISSN - 1364-5072

DOI - 10.1111/jam.14073

Subject(s) - infectious bursal disease , immunogenicity , lactobacillus plantarum , biology , recombinant dna , immune system , microbiology and biotechnology , virology , immunology , virulence , gene , lactic acid , bacteria , biochemistry , genetics

Aim To develop an effective oral vaccine against the very virulent infectious bursal disease virus (vv IBDV ), we generated two recombinant Lactobacillus plantarum strains ( pPG 612‐ VP 2/ LP and pPG 612‐T7g10‐ VP 2/ LP , which carried the T7g10 translational enhancer) that displayed the VP 2 protein on the surface, and compared the humoral and cellular immune responses against vv IBDV in chickens. Methods and Results We genetically engineered the L. plantarum strains pPG 612‐ VP 2/ LP and pPG 612‐T7g10‐ VP 2/ LP constitutively expressing the VP 2 protein of vv IBDV . We found that the T7g10 enhancer efficiently upregulates VP 2 expression in pPG 612‐T7g10‐ VP 2/ LP . Orally administered, pPG 612‐T7g10‐ VP 2/ LP exhibited significant levels of protection (87·5%) against vv IBDV in chickens, indicating improved immunogenicity. Chickens in the pPG 612‐T7g10‐ VP 2/ LP group produced higher levels of interferons ( IFN ‐γ) and interleukins ( IL ‐2 and IL ‐4) than those in the pPG 612‐ VP 2/ LP group. CD 8+ and CD 4+ lymphocyte counts indicated greater stimulation in the pPG 612‐T7g10‐ VP 2/ LP group (13·3 and 21·0% respectively) than in the pPG 612‐ VP 2/ LP group (10·4 and 14·0% respectively). Thus, pPG 612‐T7g10‐ VP 2/ LP could induce strong humoral and cellular immune responses against vvIBDV. Conclusions The recombinant L. plantarum that expresses pPG 612‐T7g10‐ VP 2 is a promising candidate for oral vaccine development against vv IBDV . Significance and Impact of the Study The recombinant Lactobacillus delivery system provides a promising strategy for vaccine development against vv IBDV in chickens.

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