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Immunogenicity and protective efficacy of orally administered recombinant Lactobacillus plantarum expressing VP 2 protein against IBDV in chicken
Author(s) -
Maqsood I.,
Shi W.,
Wang L.,
Wang X.,
Han B.,
Zhao H.,
Nadeem A.M.,
Moshin B.S.,
Saima K.,
Jamal S.S.,
Din M.F.,
Xu Y.,
Tang L.,
Li Y.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14073
Subject(s) - infectious bursal disease , immunogenicity , lactobacillus plantarum , biology , recombinant dna , immune system , microbiology and biotechnology , virology , immunology , virulence , gene , lactic acid , bacteria , biochemistry , genetics
Abstract Aim To develop an effective oral vaccine against the very virulent infectious bursal disease virus (vv IBDV ), we generated two recombinant Lactobacillus plantarum strains ( pPG 612‐ VP 2/ LP and pPG 612‐T7g10‐ VP 2/ LP , which carried the T7g10 translational enhancer) that displayed the VP 2 protein on the surface, and compared the humoral and cellular immune responses against vv IBDV in chickens. Methods and Results We genetically engineered the L. plantarum strains pPG 612‐ VP 2/ LP and pPG 612‐T7g10‐ VP 2/ LP constitutively expressing the VP 2 protein of vv IBDV . We found that the T7g10 enhancer efficiently upregulates VP 2 expression in pPG 612‐T7g10‐ VP 2/ LP . Orally administered, pPG 612‐T7g10‐ VP 2/ LP exhibited significant levels of protection (87·5%) against vv IBDV in chickens, indicating improved immunogenicity. Chickens in the pPG 612‐T7g10‐ VP 2/ LP group produced higher levels of interferons ( IFN ‐γ) and interleukins ( IL ‐2 and IL ‐4) than those in the pPG 612‐ VP 2/ LP group. CD 8+ and CD 4+ lymphocyte counts indicated greater stimulation in the pPG 612‐T7g10‐ VP 2/ LP group (13·3 and 21·0% respectively) than in the pPG 612‐ VP 2/ LP group (10·4 and 14·0% respectively). Thus, pPG 612‐T7g10‐ VP 2/ LP could induce strong humoral and cellular immune responses against vvIBDV. Conclusions The recombinant L. plantarum that expresses pPG 612‐T7g10‐ VP 2 is a promising candidate for oral vaccine development against vv IBDV . Significance and Impact of the Study The recombinant Lactobacillus delivery system provides a promising strategy for vaccine development against vv IBDV in chickens.