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Bifidobacterium animalis ssp . lactis Bb12 induces IL ‐10 through cell membrane‐associated components via TLR 2 in swine
Author(s) -
ArenasPadilla M.,
DuarteGutiérrez J.L.,
MataHaro V.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14069
Subject(s) - tlr2 , bifidobacterium animalis , microbiology and biotechnology , biology , mesenteric lymph nodes , immune system , bifidobacterium , immunology , bacteria , innate immune system , lactobacillus , genetics
Aim To investigate the role of Toll‐like receptor 2 ( TLR 2) in interleukin‐10 ( IL ‐10) production induced by Bifidobacterium animalis ssp. lactis Bb12 (Bb12) in swine immune cells. Methods and Results Blood‐monocytes and cells from mesenteric lymph nodes were obtained from pigs and cultured with live Bb12 for 4 and 12 h. Transcript levels of IL ‐10 and TLR 2 were analysed. Furthermore, TLR 2 was blocked to determine its participation in IL ‐10 production. TLR 2 blockade was achieved with neutralizing antibodies, followed by stimulation with Bb12. Bifidobacteria induced IL ‐10 production in both swine monocytes and mesenteric cells. Monocytes with TLR 2 blockade had a decrease in IL ‐10 transcripts, while mesenteric cells did not. Bacterial cell wall components were responsible for Bb12‐induced IL ‐10 production since no IL ‐10 was detected in the culture supernatant. Conclusions We demonstrated that IL ‐10 production is largely mediated through the recognition of Bb12 structures by TLR 2, as bacterial metabolites in the culture supernatant failed to induce IL ‐10 expression. Significance and Impact of the Study The present study provides evidence for the potential use of Bb12 in the swine industry; these bacteria can also be used as additional method to treat intestinal inflammation and enhance intestinal health in pigs.