Premium
Identifying the core bacterial and fungal communities within four agricultural biobeds used for the treatment of pesticide rinsates
Author(s) -
Bergsveinson J.,
Perry B.J.,
Sheedy C.,
Braul L.,
Reedyk S.,
Gossen B.D.,
Yost C.K.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14051
Subject(s) - pesticide , biology , microbial population biology , fungicide , fungal diversity , 16s ribosomal rna , bioremediation , agriculture , microbial ecology , bacteria , ecology , microbiology and biotechnology , toxicology , botany , contamination , genetics
Aims This study evaluated the variability of bacterial and fungal communities within unique pesticide remediation biobeds. Methods and Results Four biobeds receiving different applied pesticide rinsates, were sampled throughout an operational season to determine pesticide removal efficacy and microbial communities. Biomixture samples collected from different biobed depths, were subjected to Illumina sequencing of the 16S rRNA (bacteria) and ITS 2 (fungi) genes. Pesticide removal rates for all biobeds averaged 99%, with microbial community analysis revealing biobeds shared 60–70% of the most abundant bacterial and fungal orders, respectively. Though biobed depth did not greatly impact microbial community profile or diversity, bacterial and fungal taxa profiles between biobeds notably diverge at levels of genera and OTU . Biobed bacterial communities exhibited greater diversity than fungal communities between and within all biobeds. Conclusions Biobeds receiving variable pesticide rinsates share a ‘core’ microbial community, exhibiting greater bacterial diversity relative to fungal diversity. Pesticide exposure increased bacterial diversity throughout the biobeds, while fungal diversity was variable, meriting further understanding of fungicide application to biobed fungal community stability. Significance and Impact of the Study Biobeds achieve high treatment efficacy of unique pesticide rinsates, regardless of differentiation of specific genera in response to specific compounds; supporting biobeds as a robust engineered system for pesticide rinsates bioremediation.