Premium
Halobacteriovorax isolated from marine water of the Adriatic sea, Italy, as an effective predator of Vibrio parahaemolyticus, non‐O1/O139 V. cholerae, V. vulnificus
Author(s) -
Ottaviani D.,
Chierichetti S.,
Angelico G.,
Forte C.,
Rocchegiani E.,
Manuali E.,
Leoni F.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14027
Subject(s) - vibrio vulnificus , vibrio cholerae , vibrio parahaemolyticus , vibrionaceae , biology , microbiology and biotechnology , predator , marine bacteriophage , ecology , bacteria , predation , genetics
Aim To detect marine Bdellovibrio and like organisms ( BALO s) which are able to infect Vibrio parahaemolyticus from seawater of the Adriatic, Italy. To test, prey specificity and predation efficiency of our Halobacteriovorax isolate, named HBXCO 1, towards 17 Vibrio and 7 non‐ Vibrio strains linked to the Adriatic sea, Italy. Methods and Results Double layer agar plating technique was used to enumerate BALO s and to evaluate their prey specificity and predation efficiency. Transmission electron microscopy and 16S rRNA analysis were used to identify them. Means of BALO s counts ranged from 5·0 PFU per ml (March 2017) to 98·6 PFU per ml (August 2016). HBXCO 1 had the ability to attack all tested prey strains of V. parahaemolyticus , Vibrio cholerae non‐O1/O139 and Vibrio vulnificus , but it did not prey on non‐Vibrio strains and V. alginolyticus under the tested conditions. Conclusions Bdellovibrio and like organisms capable of infecting pathogenic vibrios are naturally present in seawater of the Adriatic, Italy. Isolate HBXCO 1 shows prey specificity preferentially for the Vibrio genus and high predatory efficiency towards a wide range of pathogenic strains. Significance and Impact of the Study The public impact of V. parahaemolyticus, non‐O1/O139 V. cholerae and V. vulnificus in bivalves is relevant and current decontamination processes are not always effective. We believe that the predator HBXCO 1 represents a potential candidate for the development of strategies of biocontrol of pathogenic vibrios in bivalves from harvesting to trade.