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Use of polyvalent bacteriophages to combat biofilm of Proteus mirabilis causing catheter‐associated urinary tract infections
Author(s) -
Maszewska A.,
Zygmunt M.,
Grzejdziak I.,
Różalski A.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.14026
Subject(s) - proteus mirabilis , microbiology and biotechnology , biofilm , proteus , urinary system , urinary catheter , biology , medicine , bacteria , pseudomonas aeruginosa , escherichia coli , gene , endocrinology , biochemistry , genetics
Abstract Aims Catheter‐associated urinary tract infections ( CAUTI ) caused by Proteus mirabilis are very difficult to treat due to the ability of biofilm formation and drug resistance of these bacteria. The aim of this study was to assess the antibiofilm activity of phages and develop phage cocktail to combat biofilm of P. mirabilis strains. Methods and Results Planktonic forms and biofilms of 50 tested uropathogenic P. mirabilis strains showed different sensitivity to 13 phages used. Phages 39 AP mC32, 65 AP m2833 and 72 AP m5211 presenting strong antibiofilm activity were selected as cocktail components. The antibiofilm activity of phage cocktails was similar or slightly higher than that of the most active phage. A three‐phage cocktail inhibited biofilm formation and destroyed biofilms of the same number of strains or 2–3 strains more compared to a single phage. The components of the three‐phage cocktail did not block each other's activity. Conclusions The potential of developed anti‐ P. mirabilis phage cocktail as an antibiofilm agent was proved. Significance and Impact of the Study In this study, three broad host range phages presenting strong anti‐ P. mirabilis biofilm activity were selected. Additionally, high stability of these viruses makes them a useful tool for controlling the biofilms.