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Characterization of Pseudomonas syringae pv. actinidiae biovar 3 on kiwifruit in north‐west Portugal
Author(s) -
Garcia E.,
Moura L.,
Abelleira A.,
Aguín O.,
Ares A.,
Mansilla P.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13943
Subject(s) - biovar , pseudomonas syringae , biology , actinidia , housekeeping gene , population , actinidia deliciosa , microbiology and biotechnology , botany , gene , genetics , gene expression , demography , sociology
Aims Bacterial kiwifruit canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa) was detected in north‐west Portugal in 2010, and has since caused significant losses. The objectives of this work were to characterize the Portuguese population(s) of Psa and to define the actual prevalence of Psa biovars in the most productive kiwifruit region in Portugal. Methods and Results Isolates obtained from Actinidia deliciosa orchards were characterized by morphological, biochemical, physiological, fatty acids and molecular tests ( PCR , BOX ‐ PCR , duplex‐ PCR , multiplex‐ PCR and RFLP ), phaseolotoxin, housekeeping and effector genes and pathogenicity. Results established that only Psa biovar 3 is present in the north‐west of Portugal, despite phenotypic and genetic variability among the isolates. Conclusions This work provides new information on P. syringae pv. actinidiae (Psa) genetic profile in Portugal, indicating for the first time, that two genetically different subpopulations of Psa biovar 3 are present. Significance and Impact of the Study A new subpopulation of Psa biovar 3 was found for the first time in Portugal, contributing to increase knowledge about this population worldwide and to support further understanding of the impact of Psa.