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Effect of electron beam and gamma radiation on drug‐susceptible and drug‐resistant Listeria monocytogenes strains in salmon under different temperature
Author(s) -
Skowron K.,
Grudlewska K.,
Gryń G.,
Skowron K.J.,
Świeca A.,
Paluszak Z.,
Zimek Z.,
Rafalski A.,
GospodarekKomkowska E.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13902
Subject(s) - listeria monocytogenes , microbiology and biotechnology , bacteria , antibiotics , antibiotic resistance , food irradiation , ampicillin , biology , population , food science , ionizing radiation , irradiation , medicine , genetics , physics , environmental health , nuclear physics
Aims To investigate the effect of gamma radiation and high energy electron beam doses on the inactivation of antibiotic‐susceptible and antibiotic‐resistant Listeria monocytogenes strains inoculated on the surface of raw salmon fillets stored at different temperature (−20, 4 and 25°C). Methods and Results The population of bacteria strains resistance to penicillin, ampicillin, meropenem, erythromycin and trimethoprim‐sulfamethoxazole was generated. When using gamma irradiation, the theoretical lethal dose ranged from 1·44 to 5·68 kG y and for electron beam the values ranged from 2·99 to 6·83 kG y. The theoretical lethal dose for both radiation methods was higher for antibiotic‐resistant strains. Gamma radiation proved to be a more effective method for extending salmon fillet shelf‐life. The evaluation of pulsed‐field gel electrophoresis electrophoregram revealed that the repair of radiation‐caused DNA damage occurred faster in antibiotic‐resistant L. monocytogenes strains. The number of live L. monocytogenes cells, 40 h after irradiation, also was higher in antibiotic‐resistant strain suspension. Conclusions The present study showed that gamma radiation was more effective in the elimination of the tested micro‐organisms and food preservation, than a high energy electron beam. The antibiotic‐resistant L. monocytogenes strains were more resistant to both radiation methods. Significance and Impact of the Study There are a lot of research on the effect of radiation on the number of bacteria in food products. However, there is almost no information about the effect of strain properties, such as drug susceptibility, virulence, etc., on their resistance to ionizing radiation. An increasing number of drug resistant bacterial strains isolated from food, encourages to take up this research subject.