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Characterization and antimicrobial activity of silver nanoparticles mycosynthesized by Aspergillus brasiliensis
Author(s) -
Omran B.A.,
Nassar H.N.,
Fatthallah N.A.,
Hamdy A.,
ElShatoury E.H.,
ElGendy N.Sh.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13776
Subject(s) - silver nanoparticle , silver nitrate , dynamic light scattering , crystallinity , nuclear chemistry , chemistry , scanning electron microscope , zeta potential , transmission electron microscopy , particle size , fourier transform infrared spectroscopy , materials science , analytical chemistry (journal) , nanoparticle , nanotechnology , chemical engineering , chromatography , crystallography , engineering , composite material
Aims Since mycosynthesis of metal nanoparticles (NPs) is advertised as a promising and ecofriendly approach. Thus, this study aims to investigate the capability of Aspergillus brasiliensis ATCC 16404 for mycosynthesis of silver NPs (Ag NP s). Methods and Results One‐factor‐at‐a‐time‐technique was used to study the effect of different physicochemical parameters: the reaction time, pH , temperature, different stirring rates, illumination, and finally, the different concentrations of silver nitrate and fungal biomass on the mycosynthesis of Ag NP s. The visual observation showed the characteristic brown colour formation due to the bioreduction of Ag + ions to Ag 0 by the mycelial cell‐free filtrate ( MCFF ). The UV /visible spectrophotometric technique displayed a characteristic sharp peak at ʎ 440 confirming the mycosynthesis of Ag NP s. The zeta potential value −16·7 mV assured the long‐term stability of Ag NP s and the dynamic light scattering analysis revealed good dispersion and average particle size 77 nm. The energy dispersive X‐ray spectroscopy displayed a maximum elemental distribution of silver elements. The X‐ray diffraction spectroscopy demonstrated the crystallinity of the mycosynthesized Ag NP s. The field emission scanning electron microscope and high‐resolution transmission electron microscope revealed monodispersed spherical shaped Ag NP s with average particle size of 6–21 nm. The FTIR analysis showed the major peaks of proteins providing the possible role of MCFF in the synthesis and stabilization of the Ag NP s. The mycosynthesized Ag NP s expressed good biocidal activity against different pathogenic micro‐organisms causing some water‐related diseases and health problems to local residents. Conclusions This study proved that A. brasiliensis ATCC 16404 MCFF has good potential for mycosynthesis of Ag NP s, which exhibited good antimicrobial effect on different pathogenic micro‐organisms; thus, it can be applied for water disinfection. Significance and Impact of the Study This research provides a helpful insight into the development of a new mycosynthesized antimicrobial agent.