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A strain of Streptomyces sp. isolated from rhizospheric soil of Crataegus oxycantha producing nalidixic acid, a synthetic antibiotic
Author(s) -
Arora N.,
Kumar S.,
Satti N.K.,
Ali A.,
Gupta P.,
Katoch M.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13736
Subject(s) - nalidixic acid , crataegus , strain (injury) , microbiology and biotechnology , biology , rhizosphere , antibiotics , botany , bacteria , antibiotic resistance , genetics , anatomy
Aim Exploration of microbes isolated from rhizospheric soil of Crataegus oxycantha for bioactive natural products. Methods and Results A strain of Streptomyces sp. (C‐7) was isolated from rhizospheric soil of C. oxycantha . The 16S rRNA gene sequence of strain C‐7 displayed 99% sequence similarity with different Streptomyces species. The highest score was displayed for Streptomyces sp. strain Chy2‐8 followed by Streptomyces violarus strain NBRC 13104 and Streptomyces arenae strain ISP 5293. The position of C‐7 in the phylogenetic tree suggested uniqueness of the strain. Nalidixic acid ( 1 ), a quinolone antibiotic, was isolated from Streptomyces sp. strain (C‐7) for the first time and characterized by NMR and chemically analysed. Compound 1 exhibited antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa . The production of compound 1 was also validated by repeating fermentation of strain C‐7 and compound isolation in a separate natural product laboratory with no prior information. Furthermore, Compound 1 showed a cytotoxic effect against human prostate cancer cell line PC 3 with an IC 50 11 μ g ml −1 . Conclusion To the best of our knowledge, this is the first report showing production of nalidixic acid naturally by a strain of Streptomyces sp. Significance and Impact of the Study In this study, we isolated a strain of Streptomyces sp. producing nalidixic acid, which was otherwise only obtained through chemical synthesis.