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Photodynamic inactivation of foodborne bacteria by eosin Y
Author(s) -
Bonin E.,
Santos A.R.,
Fiori da Silva A.,
Ribeiro L.H.,
Favero M.E.,
CampanerutSá P.A.Z.,
Freitas C.F.,
Caetano W.,
Hioka N.,
Mikcha J.M.G.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13727
Subject(s) - staphylococcus aureus , bacillus cereus , eosin , escherichia coli , salmonella enterica , microbiology and biotechnology , pseudomonas aeruginosa , bacteria , chemistry , eosin y , enterobacteriaceae , biology , staining , biochemistry , genetics , photocatalysis , gene , catalysis
Aims The aim of this study was evaluate the effect of photodynamic inactivation mediated by eosin Y in Salmonella enterica serotype Typhimurium ATCC 14028, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 11778. Methods and Results Bacteria (10 7 CFU per ml) were incubated with eosin Y at concentrations ranging from 0·1 to 10 μ mol l −1 , irradiated by green LED ( λ max 490–570 nm) for 5, 10 and 15 min and the cellular viability was determined. Pseudomonas aeruginosa was completely inactivated when treated with 10 μ mol l −1 eosin Y for 10 min. Treatments reduced B. cereus and Salm . Typhimurium counts to 2·7 log CFU per ml and 1·7 log CFU per ml, respectively . Escherichia coli counts were slightly reduced. Staphylococcus aureus presented the highest sensitivity, being completely inactivated by eosin Y at 5 μ mol l −1 and 5 min of illumination. The reduction of cellular viability of photoinactivated Staph. aureus was also demonstrated by flow cytometry and morphological changes were observed by scanning electron microscopy. Conclusions Eosin Y in combination with LED produced bacterial inactivation, being a potential candidate for photodynamic inactivation. Significance and Impact of the Study This study evidenced the efficacy of photodynamic inactivation as a novel and promising alternative to bacterial control.