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Antimicrobial and cytotoxic activity of silver nanoparticles synthesized from two haloalkaliphilic actinobacterial strains alone and in combination with antibiotics
Author(s) -
Wypij M.,
Świecimska M.,
Czarnecka J.,
Dahm H.,
Rai M.,
Golinska P.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13723
Subject(s) - antimicrobial , microbiology and biotechnology , minimum inhibitory concentration , silver nanoparticle , candida albicans , biofilm , chemistry , tetracycline , minimum bactericidal concentration , hela , crystal violet , staphylococcus aureus , bacteria , mtt assay , escherichia coli , biology , antibiotics , biochemistry , in vitro , materials science , nanoparticle , nanotechnology , genetics , gene
Aims Presently, the effective antimicrobial agents have been limited by the emergence of microbial strains with multidrug resistance and biofilm formation potential. In the present study, we report remarkable antimicrobial activity of silver nanoparticles (Ag NP s) synthesized from Streptomyces calidiresistens IF 11 and IF 17 strains, including inhibition of biofilm formation and synergistic effect of Ag NP s and antibiotics against selected bacteria and yeasts. Cytotoxic effect of Ag NP s on mammalian cell lines was also evaluated. Methods and Results Analysis of biosynthesized Ag NP s by Fourier Transform Infrared Spectroscopy and transmission electron microscopy revealed their spherical shape, small size in the range of 5–50 and 5–20 nm, respectively, as well as the presence of capping agents. Study of antimicrobial activity of Ag NP s against Bacillus subtilis , Staphylococcus aureus , Escherichia coli, Candida albicans and Malassezia furfur evaluated by minimum inhibitory concentration ( MIC ) and minimum biocidal concentration ( MBC ) assays revealed that MIC s of Ag NP s from IF 11 and IF 17 strains against bacteria and yeasts were found to be in the range of 16–128 and 8–256 μ g ml −1 , while MBC s were in the range of 48–192 and 32–256 μ g ml −1 respectively. Ag NP s inhibited biofilm formation of microbial strains, which was tested by using crystal violet stain. The highest synergistic effect determined by fractional inhibitory index of Ag NP s with antibiotic (kanamycin or tetracycline) was found against Staph. aureus ; while in case of yeasts, M. furfur showed highest sensitivity to Ag NP s‐ketoconazole combination ( FIC = 0·12). The cytotoxic activity of Ag NP s towards HeLa and 3T3 cell lines was studied by MTT assay. The IC 50 of Ag NP s estimated against mouse fibroblasts was found to be 8·3 and 28·3 μ g ml −1 and, against HeLa cell line, 28·5 and 53·8 μ g ml −1 respectively. Conclusions It can be concluded that Ag NP s synthesized from S. calidiresistens IF 11 and IF 17 strains have potential as an effective antimicrobial and cytotoxic agent, especially when used in combination with antibiotics/antifungal agents. Significance and Impact of the Study This study indicates potential application of biogenic silver nanoparticles as an antimicrobial agent in nanomedicine.